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cck 8 reagent  (Beijing CWBio)
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Beijing CWBio cck 8 reagent
circ_0119412 downregulation suppressed cervical cancer cell malignant behaviors and tumor growth in animal models. (A) The efficiency of circ_0119412 overexpression was checked by RT‐qPCR. (B) Cell proliferation affected by circ_0119412 overexpression was assessed by <t>CCK‐8</t> assay. (C) Cell migration affected by circ_0119412 overexpression was assessed by transwell assay. (D) Cell adhesion affected by circ_0119412 overexpression was assessed by MTT assay. (E) The role of circ_0119412 in vivo was assessed by animal study. *p < 0.05, **p < 0.001 relative to oe‐NC
Cck 8 Reagent, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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circ_0119412 downregulation suppressed cervical cancer cell malignant behaviors and tumor growth in animal models. (A) The efficiency of circ_0119412 overexpression was checked by RT‐qPCR. (B) Cell proliferation affected by circ_0119412 overexpression was assessed by CCK‐8 assay. (C) Cell migration affected by circ_0119412 overexpression was assessed by transwell assay. (D) Cell adhesion affected by circ_0119412 overexpression was assessed by MTT assay. (E) The role of circ_0119412 in vivo was assessed by animal study. *p < 0.05, **p < 0.001 relative to oe‐NC

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: circ_0119412 downregulation suppressed cervical cancer cell malignant behaviors and tumor growth in animal models. (A) The efficiency of circ_0119412 overexpression was checked by RT‐qPCR. (B) Cell proliferation affected by circ_0119412 overexpression was assessed by CCK‐8 assay. (C) Cell migration affected by circ_0119412 overexpression was assessed by transwell assay. (D) Cell adhesion affected by circ_0119412 overexpression was assessed by MTT assay. (E) The role of circ_0119412 in vivo was assessed by animal study. *p < 0.05, **p < 0.001 relative to oe‐NC

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Over Expression, Quantitative RT-PCR, CCK-8 Assay, Migration, Transwell Assay, MTT Assay, In Vivo

miR‐217 upregulation reversed the effects caused by circ_0119412 overexpression. (A) The expression of miR‐217 in Hela and SiHa cells transfected with oe‐circ, miR‐217 mimic, or oe‐circ+miR‐217 mimic was checked by RT‐qPCR. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were monitored by CCK‐8, transwell, or MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05, && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐circ+mimic

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: miR‐217 upregulation reversed the effects caused by circ_0119412 overexpression. (A) The expression of miR‐217 in Hela and SiHa cells transfected with oe‐circ, miR‐217 mimic, or oe‐circ+miR‐217 mimic was checked by RT‐qPCR. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were monitored by CCK‐8, transwell, or MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05, && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐circ+mimic

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Over Expression, Expressing, Transfection, Quantitative RT-PCR, Migration, CCK-8 Assay, MTT Assay

miR‐217 inhibited cervical cancer cell malignant behaviors by degrading AGR2. (A) The expression of AGR2 protein in Hela and SiHa cells transfected with oe‐AGR2, miR‐217 mimic, or oe‐AGR2+miR‐217 mimic was measured by western blot. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were investigated by CCK‐8, transwell, and MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05 and && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐AGR2+mimic

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: miR‐217 inhibited cervical cancer cell malignant behaviors by degrading AGR2. (A) The expression of AGR2 protein in Hela and SiHa cells transfected with oe‐AGR2, miR‐217 mimic, or oe‐AGR2+miR‐217 mimic was measured by western blot. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were investigated by CCK‐8, transwell, and MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05 and && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐AGR2+mimic

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Expressing, Transfection, Western Blot, Migration, CCK-8 Assay, MTT Assay